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DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses
 

DNA Extraction from Paraffin Embedded Material for Genetic and Epigenetic Analyses

Article DOI: 10.3791/2763-v • 13:33 min • March 26th, 2011
March 26th, 2011 •
*1,2, *1,2, 3, 1,2,4
1Department of Integrative Oncology, BC Cancer Research Centre, 2Interdisciplinary Oncology Program, University of British Columbia - UBC, 3Photography/Video Production, Multi-Media Services, BC Cancer Agency, 4Department of Pathology and Laboratory Medicine, University of British Columbia - UBC
* These authors contributed equally

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This video demonstrates the protocol for DNA extraction from formalin-fixed paraffin-embedded material. This is a multi-day procedure in which tissue sections are deparaffinized with xylene, rehydrated with ethanol and treated with proteinase K to purify and isolate DNA for subsequent gene-specific or genome-wide analysis.

Tags

DNA Extraction Paraffin Embedded Material Genetic Analysis Epigenetic Analysis Chromosomal Rearrangements Copy Number Gains And Losses DNA Methylation High-throughput Profiling Technologies Microarrays Sample Quality Specimen Collection Formalin-fixed And Paraffin Embedded (FFPE) Hospital Archives Extraction Method Tissue Cores Microdissected Tissue Xylene Treatment Ethanol Washes Proteinase K Lysis Buffer
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