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植入前胚胎,牛,羊,山羊的冷冻保存
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
DOI:

11:10 min

August 05, 2011

Chapitres

  • 00:05Titre
  • 02:28Evaluating Suitability of Embryos for Cryopreservation
  • 03:58Washing Embryos to Reduce Likelihood of Disease Transmission
  • 05:20Dehydrating Embryos and Loading Embryos into a 0.25 ml Plastic Straw
  • 07:11Freezing Embryos
  • 08:53Thawing Cryopreserved Embryos
  • 10:26Results of Transferring Frozen-thawed Embryos to Female Recipients
  • 10:45Conclusion

Summary

Traduction automatique

植入前胚胎可冷冻保存,放置到一个高渗cryoprotective溶液后,引起细胞脱水。平衡后,冰晶体的形成是诱导胚胎周围的解决方案。进一步脱水发生胚胎慢慢冷却到零度以下的温度中,前陷入液氮存储。

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