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Chapters
- 00:05Title
- 02:28Evaluating Suitability of Embryos for Cryopreservation
- 03:58Washing Embryos to Reduce Likelihood of Disease Transmission
- 05:20Dehydrating Embryos and Loading Embryos into a 0.25 ml Plastic Straw
- 07:11Freezing Embryos
- 08:53Thawing Cryopreserved Embryos
- 10:26Results of Transferring Frozen-thawed Embryos to Female Recipients
- 10:45Conclusion
Preimplantation embryos may be cryopreserved after placement into a hypertonic cryoprotective solution to cause cellular dehydration. After equilibration, ice crystal formation is induced in the solution surrounding the embryo. Further dehydration occurs as the embryo is slowly cooled to subzero temperatures before plunging into liquid nitrogen for storage.
