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In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion
Journal JoVE
Biologie
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Journal JoVE Biologie
In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion

In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion

DOI:
10.3791/3138-v

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12:35 min

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November 10, 2011

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,
1Department of Biochemistry,University of Nebraska, Lincoln

Chapitres

  • 00:05Titre
  • 02:37Purification of Hepatocytes and SECs from Rat Liver
  • 04:18Internalization of 125I-SA-b-Hep or Other Suitable Labeled Ligand
  • 06:55Hepatocyte and Sinusoidal Endothelial Cell (SEC) Purification
  • 11:08Distribution of Biotin-Labeled Heparin
  • 12:06Conclusion

Summary

Traduction automatique

Traduction automatique

The study of liver sinusoidal endothelial cells (SECs) must be performed with primary cells obtained from the animal as no cell lines exist. This method relies on liver digestion and differential centrifugation for SEC purification for subsequent culturing and experimentation.

Tags

In Vivo Liver EndocytosisPurification Of Liver CellsLiver PerfusionHepatocytesKupffer CellsStellate CellsSinusoidal Endothelial CellsFenestraeCytoplasmic HolesSpace Of DisseScavenger ReceptorsSR-AStabilin-1Stabilin-2EndocytosisPhagocytosis

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