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果蝇蛹腹部免疫

Journal JoVE
Biologie

This content is Free Access.

Journal JoVE Biologie

Drosophila Pupal Abdomen Immunohistochemistry

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果蝇蛹腹部免疫

DOI:

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08:29 min

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October 02, 2011

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Wei Wang, John H. Yoder

¹Department of Biological Sciences,University of Alabama

Chapitres

00:05Titre
01:19Collection, Culturing and Staging Pupae
02:33Dissection
03:40Cleaning, Fixation and Primary Antibody Incubation
05:38Secondary Antibody Incubation and Mounting
07:24Images of Prepared Samples
08:00Conclusion

Summary

Traduction automatique

抗体染色<em>果蝇</em>蛹可以提高成人腹部发育遗传学的遗传分析。我们目前对我们的协议清扫，固定和抗体染色上演<em>果蝇</em>蛹腹部。

Tags

Drosophila Pupal Abdomen Immunohistochemistry Epithelial Morphogenesis Sexually Dimorphic Morphologies Imaginal Cells Histoblast Nests Larval Epidermis Adult Abdominal Segments Dorsal Cuticle Ventral Cuticle Spiracles Diploid Cells Larval Epidermal Cells Molecular Tools Genetic Pathways Adult Phenotypes Dissection Immunohistochemical Analyses

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