Journal
/
/
分离的CD133 +肝干细胞的克隆扩增
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Isolation of CD133+ Liver Stem Cells for Clonal Expansion
DOI:

12:06 min

October 10, 2011

, , , ,

Chapitres

  • 00:05Titre
  • 01:45Parenchymal and Non-parenchymal Separation from Whole Liver
  • 04:34Red Cell Lysis
  • 05:51CD45 Hematopoietic Cell Depletion from Non-parenchymal Fraction
  • 07:31Flow Cytometry Isolation of CD133 Positive Cells
  • 09:03Representative Cell Sorting and Analysis
  • 11:14Conclusion

Summary

Traduction automatique

在这里,我们描述CD133的表达肝干细胞和整体的小鼠肝,一个过程,需要组织消化,细胞富集和流式细胞仪分离肿瘤干细胞的分离。包括先进的单细胞的分离和克隆扩增的方法。

Vidéos Connexes

Read Article