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Isolation of CD133+ Liver Stem Cells for Clonal Expansion
Journal JoVE
Biologie
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Journal JoVE Biologie
Isolation of CD133+ Liver Stem Cells for Clonal Expansion

Isolation of CD133+ Liver Stem Cells for Clonal Expansion

DOI:
10.3791/3183-v

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12:06 min

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October 10, 2011

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, , , ,
1Department of Pediatrics and Pharmacology,Pennsylvania State College of Medicine, 2Department of Pharmacology,Pennsylvania State College of Medicine, 3Department of Pediatrics,University of California Los Angeles, School of Medicine

Chapitres

  • 00:05Titre
  • 01:45Parenchymal and Non-parenchymal Separation from Whole Liver
  • 04:34Red Cell Lysis
  • 05:51CD45 Hematopoietic Cell Depletion from Non-parenchymal Fraction
  • 07:31Flow Cytometry Isolation of CD133 Positive Cells
  • 09:03Representative Cell Sorting and Analysis
  • 11:14Conclusion

Summary

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Here we describe the isolation of CD133 expressing liver stem cells and cancer stem cells from whole murine liver, a process that requires tissue digestion, cell enrichment, and flow cytometry isolation. We include methods for advanced single cell isolation and clonal expansion.

Tags

CD133+ Liver Stem CellsClonal ExpansionChronic Liver InjuryHepatocytesCholangiocytesLiver CancerHepatocellular CarcinomaStem Cell IsolationSolid OrganRare Population Of CellsParenchymal FractionNon-parenchymal FractionCD45 Negative CellsEnrichmentStem Cell ProliferationImmunohistochemistryFlow Cytometry Core

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