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Chapitres
- 00:05Titre
- 01:07Cell Harvest and Lysis
- 01:55Ni-affinity Purification of Hsp104
- 03:16FPLC Purification of Hsp104
- 05:14Representative Results for Hsp104 Purification and Assessment of Disaggregation Activity
- 07:04Conclusion
Tags
PurificationHsp104Protein DisaggregaseHexameric AAA+ ProteinATP HydrolysisProtein DisaggregationRenaturationDisordered AggregatesHeat ShockCross-beta Amyloid FibrilsPrionsPhenotypic VariationPreamyloid OligomersE. Coli Orthologue ClpBHsp104 OrthologuesAnimal CellsTherapeutic AgentNeurodegenerative DiseasesMisfolding Of ProteinsToxic Preamyloid OligomersAmyloid Fibrils
