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Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence
Journal JoVE
Médecine
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Journal JoVE Médecine
Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence

Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence

DOI:
10.3791/3334-v

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07:54 min

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October 25, 2011

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, , , , , ,
1Division of Pathology,University of Edinburgh, 2HistoRx Inc.

Chapitres

  • 00:05Titre
  • 01:39Image Capture using Whole Section Scanning
  • 03:27AQUA Automated Image Analysis
  • 05:48Representative Examples of Heterogeneity of Ovarian Tumor Morphology and Biomarker Expression
  • 07:29Conclusion

Summary

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Here we describe a method to quantify molecular heterogeneity in histological sections of tumor material using quantitative immunofluorescence, image analysis, and a statistical measure of heterogeneity. The method is intended for use in clinical biomarker development and analysis.

Tags

Heterogeneity MappingProtein ExpressionTumorsQuantitative ImmunofluorescenceMorphologic HeterogeneityHistopathologistsSurgical PracticePhenotype DifferencesMolecular PhenotypeProtein Expression DifferencesCellular FunctionPrognosisGenetic HeterogeneitySub-clonesMetastatic DiseaseBiomarkersTherapeutic ResponsesHistopathologic Scoring SchemesImmunohistochemistry

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