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Growth Assays to Assess Polyglutamine Toxicity in Yeast
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Journal JoVE Biologie
Growth Assays to Assess Polyglutamine Toxicity in Yeast

Growth Assays to Assess Polyglutamine Toxicity in Yeast

DOI:
10.3791/3461-v

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09:06 min

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March 05, 2012

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1Boston Biomedical Research Institute

Chapitres

  • 00:05Titre
  • 01:25Experimental Considerations and Set-up of Yeast Cells Expressing Toxic PolyQ-expansion Proteins
  • 03:26Plating Assay
  • 04:30Spotting Assay
  • 05:25Liquid Growth Assay
  • 07:08Representative Growth Assay Results
  • 08:30Conclusion

Summary

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This manuscript describes three complementary protocols for assessing the toxicity of polyglutamine (polyQ)-expansion proteins in the yeast Saccharomyces cerevisiae. These protocols can easily be modified to monitor the toxicity of other misfolded proteins in yeast.

Tags

Growth AssaysAssessPolyglutamine ToxicityYeastProtein MisfoldingNeurodegenerative DiseasesHuntington’s DiseaseCellular ToxicityPolyQ-expansionsSpinocerebellar AtaxiasKennedy’s DiseaseModel Organism YeastCellular PathwaysExperimental SystemsHD PatientsBasic MechanismsGrowth Defects

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