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Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
 

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Article DOI: 10.3791/3736-v • 12:03 min • July 8th, 2012
July 8th, 2012 •
1, 1, 1,2
1Institute for Research in Biomedicine, Bellinzona (Switzerland), 2Dipartimento di Biologia e Genetica per le Scienze Mediche, Universitá degli Studi di Milano

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résumé
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A method to analyse the distribution of bone marrow hematopoietic progenitors in flow cytometry as well as to efficiently isolate highly purified hematopoietic stem cells (HSCs) is described. The isolation procedure is essentially based on magnetic enrichment of c-Kit+ cells and cell sorting to purify HSCs for cellular and molecular studies.

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Phenotypic Analysis Isolation Murine Hematopoietic Stem Cells Lineage-committed Progenitors Bone Marrow HSCs Blood Cell Lineages Molecular Dissection Homeostasis Hematopoiesis Oncology Regenerative Medicine Labeling Protocol Fluorescent Antibodies Electronic Gating Procedure Flow Cytometry Hematopoietic Progenitor Subsets Distribution Individual Mice Magnetic Enrichment C-Kit Expression Trabecular Osteoblasts Sinusoidal Endothelium Functional Niches N-cadherin+ Osteoblasts Receptor Tyrosine Kinase Tie2 Angiopoietin-1
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