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Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry
 

Single Cell Measurement of Dopamine Release with Simultaneous Voltage-clamp and Amperometry

Article DOI: 10.3791/3798-v • 07:30 min • November 21st, 2012
November 21st, 2012 •
1, 1, 1,2
1Department of Neuroscience, University of Florida, 2Department of Psychiatry, University of Florida

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résumé
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The amperometric technique measures dopamine release from a single cell by detecting the oxidative current produced by spontaneous dopamine oxidization. Simultaneous voltage clamp and amperometry methodology reveal the mechanistic relationship between the overall "activity" of dopamine transporter and the regulatory role of this activity on the reverse transport of dopamine.

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Single Cell Measurement Dopamine Release Voltage-clamp Amperometry Synaptic Cleft Pre-synaptic Targets Post-synaptic Targets Diffusion Extracellular Enzymes Membrane Transporters Dopamine Transporter Peri-synaptic Cleft Inward Dopamine Flux Uptake Outward Transport Efflux Of Dopamine Sulzer Et Al. Forward Activity Reverse Activity Extracellular Dopamine Homeostasis Patch Clamp Recording Amperometric Recording Millisecond Time Resolution Membrane Potential Control
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