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Immunology and Infection
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JoVE Journal Immunology and Infection
Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
 

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay

Article DOI: 10.3791/3822-v • 13:09 min • September 9th, 2012
September 9th, 2012 •
1, 2, 1,3
1Applied Bioscience Program, Faculty of Science, University of Ontario Institute of Technology, 2Nursing Program, Faculty of Health Sciences, University of Ontario Institute of Technology, 3Medical Laboratory Science Program, Faculty of Health Sciences, University of Ontario Institute of Technology

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This study describes a novel microplate assay that measures FV coagulation activity during fibrin clot formation in human plasma which has not been reported previously. The method uses a kinetic microplate reader to continuously measure the change in absorbance at 405nm during fibrin clot formation in human plasma.

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Factor V Activity Human Plasma Microplate Coagulation Assay Blood Coagulation Thrombin Fibrinogen Clot Formation Prothrombinase FV Assays Automated FV Assays Microplate Platform Enzyme-catalyzed Events Clot Lysis Platelet Aggregation Coagulation Factors FV Activity Measurement Kinetic Microplate Reader Absorbance Change At 405nm
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