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A Step-by-step Metodo per la ricostituzione di un Transporter ABC in particelle lipidiche Nanodisc
Journal JoVE
Biologie
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Journal JoVE Biologie
A Step-by-step Method for the Reconstitution of an ABC Transporter into Nanodisc Lipid Particles
DOI:

12:25 min

August 31, 2012

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Chapitres

  • 00:05Titre
  • 02:08Preparation of the Membrane Scaffold Protein, MSP
  • 03:53Preparation of the MalFGK2 Complex
  • 05:09Preparation of Phospholipids
  • 06:07Preparation of Bio-Beads & Nanodisc Reconstitution
  • 07:59Nanodisc Analysis: Native Gel Electrophoresis
  • 08:26Nanodisc Analysis: Dynamic Light Scattering (DLS)
  • 09:11Nanodisc Analysis: ATPase Measurements
  • 09:47Results: Activity of the maltose Transporter Reconstituted into Nanodiscs
  • 11:15Conclusion

Summary

Traduction automatique

Nanodiscs sono piccole particelle discoidali che incorporano proteine ​​di membrana in una piccola zona di doppio strato di fosfolipidi. Forniamo un protocollo visivo che mostra il passo-passo incorporazione del trasportatore MalFGK2 in un disco.

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