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Microfluídica endotelizado para estudiar las interacciones microvasculares en Enfermedades Hematológicas
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1Department of Pediatrics,Emory University School of Medicine, 2Wallace H. Coulter Department of Biomedical Engineering,Georgia Institute of Technology and Emory University, 3Aflac Cancer Center and Blood Disorders Service of Children’s Healthcare of Atlanta , 4Winship Cancer Institute of Emory University
Chapitres
- 00:05Titre
- 01:11Fabrication of the Endothelial Microdevice
- 03:46Polydimethylsiloxane (PDMS) Preparation
- 06:25Seeding the Microfluidic Device with Endothelial Cells
- 08:49Endothelialized Microfluidics Reveal Abnormal Microvascular Interactions after Infusion with Blood from Patients with Hematologic Disease
- 10:35Conclusion
Un método para la cultura de una monocapa de células endoteliales en toda la superficie interior en 3D de un dispositivo de microfluidos con microvascular del tamaño de los canales (<30 micras) se describe. Este<em> In vitro</em> Modelo de microvasculatura permite el estudio de las interacciones biofísicas entre las células sanguíneas, células endoteliales, y factores solubles en enfermedades hematológicas.
Tags
MicrofluidicsEndothelialized MicrofluidicsMicrovascular InteractionsHematologic DiseasesMicrofabrication TechniquesBiological ExperimentsBiochemical ExperimentsMicro- And NanoscalesHematologic ProcessesBlood Cell DeformabilityBlood Cell AggregationMicrovascular Blood FlowBlood Cell-endothelial Cell InteractionsCultured Endothelial CellsMicrocirculationBiophysical Pathophysiology
