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To Metoder til heterokaryon Dannelse til Discover HCV Restriction Faktorer
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Chapitres
- 00:05Titre
- 02:02Induction of Fusion by PEG
- 05:04Immunofluorescence to Determine Fusion Efficiency
- 07:21Co-culture and Transfection of a Fusogenic PFV Glycoprotein
- 08:24Infection Assay
- 09:33Visualization of Cell Fusion
- 10:56Results: Using Heterokaryon Formation to Assess the Influence of Dominant Restrictions on the HCV Life Cycle
- 16:23Conclusion
Vi beskriver to metoder til betinget<em> Trans</em>-Komplementering af hepatitis C virus (HCV), samling og færdiggørelsen af den fulde virale livscyklus, der er afhængige af heterokaryon dannelse. Disse teknikker er egnede til at screene for cellelinier, der udtrykker dominerende begrænsning faktorer, der regulerer produktionen af infektiøst HCV afkom.
Tags
Heterokaryon FormationHCV Restriction FactorsHCV ReplicationHost-rangeChimpanzeesHCV RNA ReplicationNon-hepatic Cell LinesMurine Cell LinesSelection ProceduresHCV Replicon ConstructsAntibiotic-selectable MarkersIn Vitro ResearchHepatoma Cell LinesViral Life CycleSpecies TropismImmunocompetent Small Animal ModelGenetic IncompatibilityHost Dependency FactorsRestriction FactorsConditional Trans-complementation MethodsSomatic Cell FusionViral Replication CycleHeterokaryonsTrans-complementationInfectious Viral ProgenySubgenomic HCV Rep
