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Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection
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Neurosciences
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Journal JoVE Neurosciences
Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection

Expansion of Embryonic and Adult Neural Stem Cells by In Utero Electroporation or Viral Stereotaxic Injection

DOI:
10.3791/4093-v

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19:45 min

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October 06, 2012

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, ,
1DFG – Research Center and Cluster of Excellence for Regenerative Therapies Dresden, Germany

Chapitres

  • 00:00Titre
  • 01:55In Utero Electroporation
  • 07:40Result I
  • 08:31Viral Stereotaxic Injection
  • 08:49a) Viral Preparation
  • 13:15b) Stereotaxic Injection
  • 18:26Result II

Summary

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Controlling the expansion of somatic stem cells is a major factor hampering their study and use in therapy. Here we describe a system to temporally control neural stem cells expansion during development and adulthood, which can be used to increase the number of neurons generated in the mouse brain.

Tags

Embryonic Neural Stem CellsAdult Neural Stem CellsIn Utero ElectroporationViral Stereotaxic InjectionSomatic Stem CellsRegenerative MedicineStem Cell ExpansionStem Cell DifferentiationCdk4/cyclinD1 ComplexG1 Phase Of The Cell Cycle

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