Journal
/
Biologie
/
Electricity-Free, Sequential Nucleic Acid and Protein Isolation
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Electricity-Free, Sequential Nucleic Acid and Protein Isolation

Electricity-Free, Sequential Nucleic Acid and Protein Isolation

DOI:
10.3791/4202-v

•

09:52 min

•

May 15, 2012

•

,
1CUBRC, Inc., 2Department of Microbiology and Immunology,State University of New York at Buffalo, School of Medicine and Biomedical Sciences

Chapitres

  • 00:05Titre
  • 02:20Sample Lysis
  • 03:20Nuclei Acid Extraction
  • 05:27Protein Extraction
  • 07:11Results: Sequential Nucleic Acid and Protein Isolation
  • 09:28Conclusion

Summary

Traduction automatique

Traduction automatique

A tool and chemistries are described to sequentially isolate nucleic acids followed by proteins from a sample without the need for electricity. The tool consists of a sorbent held within a transfer pipette while the isolation chemistries are based on solid-phase extraction principles. The isolated macromolecules can be analyzed by immuno-based and PCR-based assays.

Tags

Electricity-freeSequentialNucleic Acid IsolationProtein IsolationTraditional PathogensEmerging PathogensEnterohemorrhagic Escherichia Coli (EHEC)Yersinia PestisPrion-based DiseasesConcernGovernmentsIndustriesMedical ProfessionalsDeathsChildrenDeveloping WorldLaboratory-based IdentificationUnited StatesLow CostField-based ToolsPoint-of-careRapid IdentificationDiagnosisDisease MarkersDisease ProgressionPatient PrognosisSolid-phase Extraction (SPE)Nucleic AcidsProteinsSample Isolation ToolBOOM TechnologyChaotropic Salt Solution

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/4202?article_type=v

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
check_url/fr/4202?article_type=v

Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery
check_url/fr/4202?article_type=v

NanoDrop Microvolume Quantitation of Nucleic Acids
check_url/fr/4202?article_type=v

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry
check_url/fr/4202?article_type=v

Analyzing and Building Nucleic Acid Structures with 3DNA
check_url/fr/4202?article_type=v

Protein Isolation from the Developing Embryonic Mouse Heart Valve Region
check_url/fr/4202?article_type=v

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
check_url/fr/4202?article_type=v

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
check_url/fr/4202?article_type=v

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
check_url/fr/4202?article_type=v

Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization

Read Article