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Electricity-Free, Sequential Nucleic Acid and Protein Isolation
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Electricity-Free, Sequential Nucleic Acid and Protein Isolation

Electricity-Free, Sequential Nucleic Acid and Protein Isolation

DOI:
10.3791/4202-v

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09:52 min

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May 15, 2012

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,
1CUBRC, Inc., 2Department of Microbiology and Immunology,State University of New York at Buffalo, School of Medicine and Biomedical Sciences

Chapters

  • 00:05Title
  • 02:20Sample Lysis
  • 03:20Nuclei Acid Extraction
  • 05:27Protein Extraction
  • 07:11Results: Sequential Nucleic Acid and Protein Isolation
  • 09:28Conclusion

Summary

Automatic Translation

Automatic Translation

A tool and chemistries are described to sequentially isolate nucleic acids followed by proteins from a sample without the need for electricity. The tool consists of a sorbent held within a transfer pipette while the isolation chemistries are based on solid-phase extraction principles. The isolated macromolecules can be analyzed by immuno-based and PCR-based assays.

Tags

Electricity-freeSequentialNucleic Acid IsolationProtein IsolationTraditional PathogensEmerging PathogensEnterohemorrhagic Escherichia Coli (EHEC)Yersinia PestisPrion-based DiseasesConcernGovernmentsIndustriesMedical ProfessionalsDeathsChildrenDeveloping WorldLaboratory-based IdentificationUnited StatesLow CostField-based ToolsPoint-of-careRapid IdentificationDiagnosisDisease MarkersDisease ProgressionPatient PrognosisSolid-phase Extraction (SPE)Nucleic AcidsProteinsSample Isolation ToolBOOM TechnologyChaotropic Salt Solution

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