Journal
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Immunologie et infection
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鞘糖脂抗原的质谱分析
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Mass Spectrometric Analysis of Glycosphingolipid Antigens
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鞘糖脂抗原的质谱分析

DOI:
10.3791/4224-v

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13:09 min

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April 16, 2013

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, ,
1Undergraduate Program,Rice University , 2Proteomics Facility, Department of Pathology,University of Texas MD Anderson Cancer Center , 3Department of Melanoma Medical Oncology,University of Texas MD Anderson Cancer Center , 4University of Texas Graduate School of Biological Sciences at Houston

Chapitres

  • 00:05Titre
  • 01:47Extraction of Glycosphingolipids from Rat Leukemia Cells
  • 02:50Separation of Neutral and Acidic Lipids
  • 05:03Removal of Phospholipids from the Neutral Fraction
  • 08:14Permethylation of Glycosphingolipids
  • 10:04Results: Mass Spectra of Permethylated Glycosphingolipids
  • 12:12Conclusion

Summary

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Traduction automatique

一个特异和敏感的方法来深入了解糖脂抗原的免疫器官和细胞的表达谱。该方法利用离子阱质谱可以逐步碎裂的糖脂分子结构分析，比较，化学​​合成的标准。

Tags

Mass Spectrometric AnalysisGlycosphingolipid AntigensGSL’sGlycoconjugate BiomacromoleculesStructural InformationEmbryonic DevelopmentSignal TransductionImmune Receptor RecognitionSugar MoietiesIsomersLipid MoietiesFatty Acyl Chain LengthUnsaturationHydroxylationCarbohydrate PortionCeramide PortionTri-hexosylceramidesGlobotriaosylceramide (Galα4Galβ4Glcβ1Cer)Isoglobotriaosylceramide (Galα3Galβ4Glcβ1Cer)Molecular MassesSugar LinkagesBiological FunctionsModification Of Ceramide PartAlpha-galactosylceramideCytokine Secretion ProfilesInvariant NKT CellsCancer ModelsAutoimmune DiseasesStructural Analysis Of IsomersImmune Organs And Cells

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