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F1FO ATPase Vesicle Preparation and Technique for Performing Patch Clamp Recordings of Submitochondrial Vesicle Membranes
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Neurosciences
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Journal JoVE Neurosciences
F1FO ATPase Vesicle Preparation and Technique for Performing Patch Clamp Recordings of Submitochondrial Vesicle Membranes

F1FO ATPase Vesicle Preparation and Technique for Performing Patch Clamp Recordings of Submitochondrial Vesicle Membranes

DOI:
10.3791/4394-v

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08:21 min

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May 04, 2013

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, , ,
1Department of Internal Medicine,Yale University

Chapitres

  • 00:05Titre
  • 02:00Brain Mitochondrial Isolation
  • 04:04Submitochondrial Vesicles Isolation
  • 05:13Electrophysiological Recording
  • 06:35Results: Patch Clamp Recordings of Submitochondrial Vesicle Membranes
  • 07:46Conclusion

Summary

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A method to isolate submitochondrial vesicles enriched in F1FO ATP synthase complexes from rat brain is described. These vesicles allow the study of the activity of F1FO ATPase complex and its modulation using the technique of patch clamp recording.

Tags

F1FO ATPaseVesicle PreparationPatch Clamp RecordingsSubmitochondrial Vesicle MembranesMitochondriaATP ProductionOxidative PhosphorylationProgrammed Cell DeathF1FO-ATP SynthaseApoptosisNecrosisBCL-2 Family ProteinsMitochondrial Outer MembranePro-apoptotic FactorsCytosolNecrotic Cell DeathMatrix CalciumMitochondrial Permeability Transition Pore (mPTP)Inner Membrane PoreBcl-xL Protein

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