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Immunology and Infection

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JoVE Journal Immunology and Infection
A Visual Assay to Monitor T6SS-mediated Bacterial Competition
 

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

Article DOI: 10.3791/50103-v • 08:45 min • March 20th, 2013
March 20th, 2013 •
1, 1, 1
1MRC Centre for Molecular Bacteriology and Infection, Division of Cell and Molecular Biology, Imperial College London

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We describe a qualitative assay to monitor bacterial competition mediated by the Pseudomonas aeruginosa type VI secretion system (T6SS). The assay relies on the survival/killing of Escherichia coli target cells carrying a lacZ-reporter. This technique is adjustable to assess the bactericidal/bacteriostasis activity of T6SS-proficient microorganisms.

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Keywords: T6SS Bacterial Competition Visual Assay Hcp VgrG Tse Tsi Pseudomonas Aeruginosa Antimicrobial Activity CFU Counting Antibiotic Resistance Screening Tool
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