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Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster
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Neurosciences
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Journal JoVE Neurosciences
Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

DOI:
10.3791/50150-v

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10:33 min

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March 04, 2013

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, , , ,
1Institute of Genetics,University of Mainz, 2Department of Anatomy and Neuroscience,University of Melbourne

Chapitres

  • 00:05Titre
  • 01:14Embryo Collection and Preparation
  • 04:21Dye Injection of Individual Neruons
  • 08:26DiI Labeled Neurons
  • 10:01Conclusion

Summary

Traduction automatique

Traduction automatique

We present a technique for labeling single neurons in the central nervous system (CNS) of Drosophila embryos, which allows the analysis of neuronal morphology by either transmitted light or confocal microscopy.

Tags

Single Cell LabelingDrosophila MelanogasterCentral Nervous SystemDiIJuxtacellular InjectionNeuronal Cell MorphologyPhotoconversionConfocal MicroscopyGenetically Encoded Fluorescent MarkersMutant Phenotypes

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