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Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens
 

Improved Protocol For Laser Microdissection Of Human Pancreatic Islets From Surgical Specimens

Article DOI: 10.3791/50231-v • 09:44 min • January 6th, 2013
January 6th, 2013 •
1,2, 3, 1,2, 1, 2, 1,2, 2, 4, 5, 6, 7, 8, 9, 2, 10, 3, 1
1Molecular Diabetology, Paul Langerhans Institute Dresden, 2Department of GI-, Thoracic- and Vascular Surgery, University Hospital Carl Gustav Carus, University of Technology Dresden, 3Department of Endocrinology and Metabolism, Metabolic Unit University of Pisa, 4Labs DC0522, Lilly Corporate Center, 5Genomics, Faculty of Medicine Imperial College London, 6Vital-IT, SIB Swiss Institute of Bioinformatics, 7Clinical Biochemistry, Hannover Medical School, 8Cell Physiology and Metabolism, Medical School, University of Geneva, 9Department of Pathology, University Hospital Carl Gustav Carus, University of Technology Dresden, 10R&D DIAB Division / Translational Medicine, Sanofi-Aventis

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Laser microdissection is a technique that allows the recovery of selected cells from minute amounts of parenchyma. Here we describe a protocol for acquiring human pancreatic islets from surgical specimens to be used for transcriptomic studies. Our protocol improves the intrinsic autofluorescence of human beta cells, thus facilitating their collection.

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Keywords: Laser Microdissection Pancreatic Islets Transcriptomics RNA Preservation Cryosectioning Autofluorescence PALM MicroBeam Tissue Handling
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