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Purification of Transcripts and Metabolites from Drosophila Heads
Journal JoVE
Biologie
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Journal JoVE Biologie
Purification of Transcripts and Metabolites from Drosophila Heads
DOI:

12:49 min

March 15, 2013

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Chapitres

  • 00:05Titre
  • 01:24Generating a Replicate and Freezing Flies
  • 02:42Collection and Lyophilization of Fly Heads
  • 03:53RNA-specific Procedures: Total RNA Extraction and mRNA Purification
  • 07:23Metabolite-specific Procedures: Methanol-chloroform Extraction
  • 09:54Representative Results
  • 12:23Conclusion

Summary

Traduction automatique

We describe here the procedures for the extraction and purification of mRNA and metabolites from Drosophila heads. We are applying these techniques to better understand the cellular perturbations underlying neuronal degeneration. These methodologies can be easily scaled and adapted for other "omic" projects.

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