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Determination of Microbial Extracellular Enzyme Activity in Waters, Soils, and Sediments using High Throughput Microplate Assays
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Environnement
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Journal JoVE Environnement
Determination of Microbial Extracellular Enzyme Activity in Waters, Soils, and Sediments using High Throughput Microplate Assays

Determination of Microbial Extracellular Enzyme Activity in Waters, Soils, and Sediments using High Throughput Microplate Assays

DOI:
10.3791/50399-v

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15:23 min

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October 01, 2013

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, ,
1Department of Biology,The University of Mississippi

Chapitres

  • 00:05Titre
  • 01:41Conducting the Enzyme Assay for Soils and Sediments
  • 05:49Setting up Water Samples, Sample, Standard, Quench, and Substrate Controls
  • 08:54Recording Fluorescence
  • 09:50Calculation of Enzyme Activity per Volume of Water
  • 10:42Results: Extracellular Enzyme Activity in Waters, Soils, and Sediments
  • 14:44Conclusion

Summary

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Microplate based procedures are described for the colorimetric or fluorometric analysis of extracellular enzyme activity. These procedures allow for the rapid assay of such activity in large numbers of environmental samples within a manageable time frame.

Tags

Microbial Extracellular Enzyme ActivityNutrient CyclingCarbon ProcessingOrganic Matter DecompositionNitrogen MineralizationPhosphorus MineralizationColorimetric AssayFluorometric AssayHigh-throughput MicroplateEcosystem Processes

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