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Immunology and Infection

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JoVE Journal Immunology and Infection
A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry
 

A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry

Article DOI: 10.3791/50410-v • 08:26 min • April 9th, 2013
April 9th, 2013 •
1, 2, 3
1Department of Biological Sciences, University of Alberta, 2División de Estudios de Posgrado e Investigación, Facultad de Odontología, Universidad Nacional Autónoma de México, 3Department of Immunology, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México

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résumé
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Neutrophils are the most abundant leukocytes in blood. Neutrophils possess transcriptionally regulated functions such as production of proinflammatory cytokines and inhibition of apoptosis. These functions can be studied with the method presented here, which allows detection and quantification of nuclear factors by flow cytometry in isolated nuclei

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Keywords: Neutrophils Leukocytes Phagocytosis Reactive Oxygen Species Cytokines Apoptosis Transcription Flow Cytometry Nuclear Factors NF-κB Elk-1
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