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A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System
 

A Chemical Screening Procedure for Glucocorticoid Signaling with a Zebrafish Larva Luciferase Reporter System

Article DOI: 10.3791/50439-v • 13:22 min • September 10th, 2013
September 10th, 2013 •
1, 1, 2, 1, 1,3, 1
1Institute of Toxicology and Genetics, Karlsruhe Institute of Technology - Campus North, 2Institute of Organic Chemistry, Karlsruhe Institute of Technology - Campus North, 3Institute of Organic Chemistry, Karlsruhe Institute of Technology - Campus South

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We describe the procedure and data analysis of a chemical screening system for glucocorticoid stress hormone signaling using zebrafish larvae: the Glucocorticoid Responsive In vivo Zebrafish Luciferase activitY (GRIZLY) assay. The assay sensitively and specifically detects effects on glucocorticoid signaling by compounds that require metabolization or affect endogenous glucocorticoid production.

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Keywords: Glucocorticoid Signaling Zebrafish Larva Luciferase Reporter System Chemical Screening GRIZLY Assay In Vivo High-throughput Screening Endocrine Disruptors Stress Research
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