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Bioengineering

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JoVE Journal Bioengineering
Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry
 

Localization and Relative Quantification of Carbon Nanotubes in Cells with Multispectral Imaging Flow Cytometry

Article DOI: 10.3791/50566-v • 14:09 min • December 12th, 2013
December 12th, 2013 •
*1, *2, 3, 1, 1, 3, 1
1Laboratoire Matière et Systèmes Complexes (MSC), CNRS/Université Paris Diderot, 2ImagoSeine BioImaging Core Facility, Institut Jacques Monod, CNRS/Université Paris Diderot, 3Laboratoire d'Immunopathologie et Chimie Thérapeutique, CNRS/Institut de Biologie Moléculaire et Cellulaire
* These authors contributed equally

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résumé
Automatic Translation

In this study, the main purpose was to monitor the cellular uptake and eventual exocytosis of carbon nanotubes (CNTs). From this perspective, we proposed here a unique method using multispectral imaging flow cytometry allowing a quantification and localization of CNTs in a statistically relevant number of cells.

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Keywords: Carbon Nanotubes CNTs Localization Quantification Multispectral Imaging Flow Cytometry ImageStream Light Absorption Light Scattering Fluorescence Pixel-by-pixel Analysis Nanomaterials Cell-nanomaterial Interactions
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