Journal
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Sex Stratified neuronale kweken om ischemische Cell Death Pathways Studie
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Sex Stratified Neuronal Cultures to Study Ischemic Cell Death Pathways
DOI:

10:44 min

December 09, 2013

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Chapitres

  • 00:05Titre
  • 01:05Preparations
  • 02:30Sexing and Dissecting E18 Embryos
  • 06:32Neuronal Dissociation
  • 07:26Plating and Culture Maintenance
  • 08:45Confirming the Sex of the Primary Culture Neurons
  • 10:22Conclusion

Summary

Traduction automatique

Primary disassociated embryonic hippocampal neuronal cultures are useful for investigating the signaling mechanisms involved in neuron death. Sexing the embryos before the isolation and dissociation of the hippocampus allows the preparation of separate male and female cultures, which enables the researcher to identify and investigate sex-specific cell signaling.

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