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Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
 

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

Article DOI: 10.3791/50762-v • 16:11 min • September 16th, 2013
September 16th, 2013 •
*1, *2, 3, 4, 2, 4
1Department of Biology, California Institute of Technology, 2Department of Bioengineering, California Institute of Technology, 3Synthetic Biology Center, Department of Bioengineering, Massachusetts Institute of Technology, 4School of Physics and Astronomy, University of Minnesota
* These authors contributed equally

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résumé
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This five-day protocol outlines all steps, equipment, and supplemental software necessary for creating and running an efficient endogenous Escherichia coli based TX-TL cell-free expression system from scratch. With reagents, the protocol takes 8 hours or less to setup a reaction, collect, and process data.

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Keywords: Cell-free Expression System Escherichia Coli TX-TL Transcription-translation Synthetic Biology Protein Expression In Vitro Endogenous T7 RNA Polymerase Lac/tet Repressors MRNA Degradation DNA Degradation Biomolecular Breadboard
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