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Slide Preparation Method to Preserve Three-dimensional Chromatin Architecture of Testicular Germ Cells
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Journal JoVE Biologie
Slide Preparation Method to Preserve Three-dimensional Chromatin Architecture of Testicular Germ Cells

Slide Preparation Method to Preserve Three-dimensional Chromatin Architecture of Testicular Germ Cells

DOI:
10.3791/50819-v

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07:34 min

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January 10, 2014

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1Division of Reproductive Sciences, Division of Developmental Biology, Perinatal Institute,Cincinnati Children’s Hospital Medical Center, 2Department of Pediatrics,University of Cincinnati College of Medicine

Chapitres

  • 00:05Titre
  • 01:543D Slide Preparation
  • 04:03Cot-1 RNA FISH
  • 06:08Results: 3D Chromatin Architecture
  • 07:09Conclusion

Summary

Traduction automatique

Traduction automatique

The material here describes a method developed to preserve the three-dimensional chromatin structure of testicular germ cells. This has been termed the three-dimensional (3D) slide method. This method improves sensitivity for detection of subnuclear structures and is applicable for immunofluorescence, DNA, and RNA fluorescence in situ hybridization (FISH).

Tags

Three-dimensional Chromatin ArchitectureTesticular Germ CellsSlide Preparation MethodPermeabilizationFixationCytospinImmunofluorescenceDNA FISHRNA FISHCot-1 RNA FISHGerm Cell Differentiation

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