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Immunology and Infection

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JoVE Journal Immunology and Infection
Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors
 
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Production and Purification of Non Replicative Canine Adenovirus Type 2 Derived Vectors

Article DOI: 10.3791/50833-v • 14:55 min • December 3rd, 2013
December 3rd, 2013 •
1, 2, 1, 2
1INSERM UMR 1043, CNRS UMR 5282, Université Toulouse 3, 2UMR Viroligie, INRA ENVA ANSES

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These past 15 years, canine adenovirus type 2 (CAV2)-derived vectors have proven their efficiency to transduce cells in vitro and in vivo and are widely used for vaccination and gene therapy. Here, we describe a procedure to construct, produce and purify CAV2 vectors, giving rise to high-titer viral suspensions.

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Keywords: Adenovirus Canine Adenovirus Type 2 (CAV2) Gene Transfer Gene Therapy Vaccine Vector Recombinant Vector Viral Production Viral Purification Cesium Chloride Gradient Viral Titer
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