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Single-cell Microinjection for Cell Communication Analysis
 

Single-cell Microinjection for Cell Communication Analysis

Article DOI: 10.3791/50836-v • 10:00 min • February 26th, 2017
February 26th, 2017 •
*1, *1, 1, 1, 1
1Institute Oswaldo Cruz, Laboratory of Cellular Communication, Oswaldo Cruz Foundation
* These authors contributed equally

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résumé
Automatic Translation

We describe here how to perform a single-cell microinjection of Lucifer Yellow to visualize cellular communication via gap-junctions in living cells, and provide some useful tips. We expect that this paper will help everyone to evaluate the degree of cellular coupling due to functional gap junctions. Everything described here could be, in principle, adapted to other fluorescent dyes with molecular weight below 1,000 Daltons.

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Keywords: Single-cell Microinjection Cell Communication Analysis P2 Receptors Gap Junctions Immune System Liver Microinjection Setup Fluorescence Microscope CCD Camera Current Generator Microelectrode Micro-manipulator Borosilicate Glass Capillary Polar Tungsten Filament Dye Loading Sodium Solution Silver Wire Head Stage Petri Dish Magnification Lens Cell Targeting Microelectrode Testing
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