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Immunology and Infection

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JoVE Journal Immunology and Infection
Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches
 

Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches

Article DOI: 10.3791/51055-v • 09:04 min • October 27th, 2013
October 27th, 2013 •
1, 1, 1, 1
1Institute of Microbiology, University Hospital Center and University of Lausanne

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Amoebal coculture is a cell culture system using adherent amoebae to selectively grow intracellular pathogens able to resist phagocytic cells such as amoebae and macrophages. It thus represents a key tool to discover new infectious agents. Amoebal enrichment allows discovery of new amoebal species and of their specific intracellular bacteria.

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Intracellular Pathogens Legionella Mycobacteria Chlamydia-like Organisms Amoebae Host-cell Bacterial Isolation Amoebal Coculture Amoebal Enrichment Clinical Samples Environmental Samples Intracellular Bacteria Amoebal Species Phagocytosis Pathogenic
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