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Immunologie et infection
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Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion (STED) Nanoscopy

DOI:
10.3791/51100-v

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10:00 min

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March 24, 2014

•

,
1Center for Human Immunobiology,Texas Children’s Hospital and Baylor College of Medicine

Chapitres

  • 00:05Titre
  • 01:25Activation of NK Cell on Antibody-coated Coverslips
  • 03:19Fixing, Permeabilizing, and Staining NK Cells on Coverslips
  • 04:52Mounting the Coverslips
  • 05:35STED Imaging
  • 08:21Optimizing STED Imaging: F-actin in NK Cells
  • 09:30Conclusion

Summary

Traduction automatique

Traduction automatique

Here we illustrate the protocol for imaging by two-color STED nanoscopy the cytotoxic immune synapse of NK cells recapitulated on glass. Using this method we obtain sub-100 nm resolution of synapse proteins and the cytoskeleton.

Tags

Immunological SynapseNatural Killer CellsActin ReorganizationSuper-resolution ImagingStimulated Emission Depletion (STED) NanoscopyTime-gated STEDCytotoxic SynapseF-actin Architecture

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