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Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling
Journal JoVE
Environnement
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Journal JoVE Environnement
Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling

Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling

DOI:
10.3791/51117-v

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10:16 min

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January 16, 2014

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, , , , , ,
1Natural Resource Ecology Laboratory,Colorado State University, 2Soil Plant Nutrient REsearch,USDA-ARS, 3Department of Soil and Crop Sciences,Colorado State University

Chapitres

  • 00:05Titre
  • 01:26Chamber Construction
  • 02:37Temperature, Humidity, and CO2 Controls
  • 04:51Irrigation System and Potting Plants
  • 06:23Fertilization with a 15N-labeled Fertilizer
  • 07:22Harvest
  • 08:14Results: Successful Incorporation of 13C and 15N into Plant Tissue
  • 09:51Conclusion

Summary

Traduction automatique

Traduction automatique

This method explains how to build and operate a continuous 13C and 15N isotope labeling chamber for uniform or differential plant tissue labeling. Representative results from metabolic and structural labeling of Andropogon gerardii are discussed.

Tags

13C15NIsotope LabelingContinuous LabelingUniform LabelingDifferential LabelingPlant MaterialEcosystem ProcessesBiogeochemical TransformationsLitter DecompositionSoil Organic MatterStructural ComponentsMetabolic Components

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