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Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling
 

Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling

Article DOI: 10.3791/51117-v • 10:16 min • January 16th, 2014
January 16th, 2014 •
1, 1, 1, 1, 1, 2, 1,3
1Natural Resource Ecology Laboratory, Colorado State University, 2Soil Plant Nutrient REsearch, USDA-ARS, 3Department of Soil and Crop Sciences, Colorado State University

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This method explains how to build and operate a continuous 13C and 15N isotope labeling chamber for uniform or differential plant tissue labeling. Representative results from metabolic and structural labeling of Andropogon gerardii are discussed.

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Keywords: 13C 15N Isotope Labeling Continuous Labeling Uniform Labeling Differential Labeling Plant Material Ecosystem Processes Biogeochemical Transformations Litter Decomposition Soil Organic Matter Structural Components Metabolic Components
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