Journal
/
Chimie
/
Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
Journal JoVE
Chimie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Chimie
Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

DOI:
10.3791/51411-v

•

12:04 min

•

March 27, 2014

•

,
1Forage-Animal Production Research Unit, Agricultural Research Service,United States Department of Agriculture

Chapitres

  • 00:05Titre
  • 01:22Preparation of Thin-layer Plates
  • 03:39Preparation of Developing Chambers for Extract Separation
  • 04:23Loading and Development of TLC Plates
  • 06:04Preparation of Plates for Bioassay
  • 07:41Bacterial Culture and Assay
  • 09:14Visualization of Bioassayed Agar Plates
  • 10:07TLC and Contact Bioautography Assay Results
  • 11:21Conclusion

Summary

Traduction automatique

Traduction automatique

Methods are described for thin-layer chromatographic (TLC) separation of plant extracts and contact bioautography to identify antibacterial metabolites. The methods are applied to the screening of red clover phenolic compounds inhibiting hyper ammonia-producing bacteria (HAB) native to the bovine rumen.

Tags

Thin-layer ChromatographyTLCBioassayPlant ExtractAntimicrobial CompoundsBioautographyMicrobial GrowthUV VisualizationTetrazolium Red StainingClostridium SticklandiiRed CloverPhenolic CompoundsHyper Ammonia-producing BacteriaRumen

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/51411?article_type=v

Profiling the Triacylglyceride Contents in Bat Integumentary Lipids by Preparative Thin Layer Chromatography and MALDI-TOF Mass Spectrometry
check_url/fr/51411?article_type=v

Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins
check_url/fr/51411?article_type=v

Analysis of Volatile and Oxidation Sensitive Compounds Using a Cold Inlet System and Electron Impact Mass Spectrometry
check_url/fr/51411?article_type=v

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical (Methylation) and Physical (Mass Spectrometry, Nuclear Magnetic Resonance) Techniques
check_url/fr/51411?article_type=v

On-line Analysis of Nitrogen Containing Compounds in Complex Hydrocarbon Matrixes
check_url/fr/51411?article_type=v

Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds
check_url/fr/51411?article_type=v

Raman and IR Spectroelectrochemical Methods as Tools to Analyze Conjugated Organic Compounds
check_url/fr/51411?article_type=v

Using Cyclic Voltammetry, UV-Vis-NIR, and EPR Spectroelectrochemistry to Analyze Organic Compounds
check_url/fr/51411?article_type=v

Electrophoretic Crystallization of Ultrathin High-performance Metal-organic Framework Membranes
check_url/fr/51411?article_type=v

Design, Synthesis, and Photochemical Properties of Clickable Caged Compounds

Read Article