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JoVE Journal Chemistry
Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds
 

Thin-layer Chromatographic (TLC) Separations and Bioassays of Plant Extracts to Identify Antimicrobial Compounds

Article DOI: 10.3791/51411-v • 12:04 min • March 27th, 2014
March 27th, 2014 •
1, 1
1Forage-Animal Production Research Unit, Agricultural Research Service, United States Department of Agriculture

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Summary
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Methods are described for thin-layer chromatographic (TLC) separation of plant extracts and contact bioautography to identify antibacterial metabolites. The methods are applied to the screening of red clover phenolic compounds inhibiting hyper ammonia-producing bacteria (HAB) native to the bovine rumen.

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Keywords: Thin-layer Chromatography TLC Bioassay Plant Extract Antimicrobial Compounds Bioautography Microbial Growth UV Visualization Tetrazolium Red Staining Clostridium Sticklandii Red Clover Phenolic Compounds Hyper Ammonia-producing Bacteria Rumen
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