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在离体血液灌注大鼠肺备量化单微血管通透性

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Biologie

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Journal JoVE Biologie

Quantifying Single Microvessel Permeability in Isolated Blood-perfused Rat Lung Preparation

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在离体血液灌注大鼠肺备量化单微血管通透性

DOI:

10.3791/51552-v

•

07:22 min

•

June 30, 2014

•

Kathirvel Kandasamy, Kaushik Parthasarathi²

¹Department of Physiology,The University of Tennessee Health Science Center, ²Department of Orthopedic Surgery and Biomedical Engineering,The University of Tennessee Health Science Center

Chapitres

00:05Titre
01:07Isolating the Rat Lung With Attached Heart
02:25Blood Perfusion of the Isolated Rat Lung
03:40Microvascular Infusion of the Lung
06:05Vascular Permeability after Bacterial Lipopolysaccharide Treatment
06:58Conclusion

Summary

Traduction automatique

孤立的血液灌注肺的准备，使可视化微血管网络上的肺表面是可行的。在这里，我们描述的方法用实时荧光成像量化单一微血管隔离肺的通透性。

Tags

Single Microvessel Permeability Isolated Blood-perfused Rat Lung Real-time Imaging Pulmonary Microvessels Permeability Changes Fluorophore Infusion Bacterial Lipopolysaccharide Venular And Capillary Microvessel Segments Lung Permeability Lung Microvascular Permeability

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