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体外胰腺器官从分散的小鼠胚胎祖细胞

Journal JoVE
Biologie

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Journal JoVE Biologie

In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors

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体外胰腺器官从分散的小鼠胚胎祖细胞

DOI:

10.3791/51725-v

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08:35 min

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July 19, 2014

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Chiara Greggio*¹, Filippo De Franceschi*¹, Manuel Figueiredo-Larsen*², Anne Grapin-Botton²

¹Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences,Swiss Institute for Experimental Cancer Research, ²DanStem,University of Copenhagen

Chapitres

00:05Titre
01:55Dissection of Dorsal Pancreatic Bud from Mouse Embryos
04:19Plating and Culturing of Dispersed Cells
06:06Results: In Vitro Expansion and Differentiation of Embryonic Pancreatic Progenitors
07:20Conclusion

Summary

Traduction automatique

在本协议中所述的三维培养方法概括胰腺发育从胚胎分散小鼠胰腺祖细胞，包括他们的大幅扩张，分化和形态发生成支器官。这种方法适合于成像，利基的功能的干扰和操纵。

Tags

Keywords: In Vitro Pancreas Organogenesis Mouse Embryonic Progenitors 3D Culture Pancreatic Progenitors Pancreas Development Pancreatic Organoids Pancreatic Morphogenesis Pancreatic Cell Differentiation Epithelial Polarization Branching Morphogenesis Mechanical Cues Cell Tensegrity

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