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Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model
 

Cell Surface Marker Mediated Purification of iPS Cell Intermediates from a Reprogrammable Mouse Model

Article DOI: 10.3791/51728-v • 10:32 min • September 6th, 2014
September 6th, 2014 •
*1,2, *1,2, 1,2, 1,2, 1,2
1Department of Anatomy and Developmental Biology, Monash University, 2Australian Regenerative Medicine Institute, Monash University
* These authors contributed equally

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résumé
Automatic Translation

Mouse embryonic fibroblast can be reprogrammed into induced pluripotent stem cells at low efficiency by the forced expression of transcription factors Oct-4, Sox-2, Klf-4, c-Myc. The rare intermediates of the reprogramming reaction are FACS isolated via labeling with antibodies against cell surface makers Thy-1.2, Ssea-1, and Epcam.

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Keywords: Cell Surface Marker IPS Cell Reprogrammable Mouse Model Induced Pluripotent Stem Cells Reprogramming Cell Surface Marker Expression Thy-1.2 Ssea-1 Epcam Mouse Embryonic Fibroblasts OKSM Doxycycline Fluorescent Activated Cell Sorting
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