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Cell Analyzer basados ​​en impedancia Real-Time como una herramienta para delinear vías moleculares implicadas en la neurotoxicidad y neuroprotección en una línea celular neuronal
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Real-Time Impedance-based Cell Analyzer as a Tool to Delineate Molecular Pathways Involved in Neurotoxicity and Neuroprotection in a Neuronal Cell Line
DOI:

08:23 min

August 09, 2014

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Chapitres

  • 00:05Titre
  • 01:39Plating and Proliferation of SK-N-SH Cells
  • 03:59Serum Deprivation
  • 05:04Data Analysis
  • 06:11Results: Representative Neurotoxicity/Neuroprotection Data
  • 07:53Conclusion

Summary

Traduction automatique

Improved in vitro neurotoxicity assays would aid the identification of new neuroprotective compounds. The utility of a real-time impedance-based cell analyzer to determine cytotoxicity and cytoprotection in neuronal cell lines and to delineate the involvement of second messenger pathways, thus gaining insight in the mechanism of neuroprotection is presented.

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