Journal
/
/
High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
High-throughput Assay to Phenotype Salmonella enterica Typhimurium Association, Invasion, and Replication in Macrophages
DOI:

11:10 min

August 11, 2014

, , , , , ,

Chapitres

  • 00:05Titre
  • 01:59Murine Macrophage RAW264.7 Cell Culture
  • 03:29Preparation of Salmonella Wild-type and Mutants
  • 05:19Invasion Assay in a 96-well Culture Plate
  • 09:58Results: Identification of the Phenotype of Salmonella Mutants
  • 10:46Conclusion

Summary

Traduction automatique

A high-throughput assay to in vitro phenotype Salmonella or other bacterial association, invasion, and replication in phagocytic cells with high-throughput capacity was developed. The method was employed to evaluate Salmonella gene knockout mutant strains for their involvements in host-pathogen interactions.

Vidéos Connexes

Read Article