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A Microfluidic-based Electrochemical Biochip for Label-free DNA Hybridization Analysis
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Chapitres
- 00:05Titre
- 02:06Microfabrication of the Electrochemical Chip
- 06:36Fabrication of the PDMS Chip
- 08:52Assembly and Preparation of the Microfluidic Arrayed Electrochemical Device
- 10:44DNA Hybridization Analysis
- 12:14Validating Electrochemical Activity of the Biochip and Detection of Hybridization Events
- 13:55Conclusion
We present a microfluidic-based electrochemical biochip for DNA hybridization detection. Following ssDNA probe functionalization, the specificity, sensitivity, and detection limit are studied with complementary and non-complementary ssDNA targets. Results illustrate the influence of the DNA hybridization events on the electrochemical system, with a detection limit of 3.8 nM.
