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Methylnitrosourea (MNU)-induced Retinal Degeneration and Regeneration in the Zebrafish: Histological and Functional Characteristics
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Journal JoVE Biologie
Methylnitrosourea (MNU)-induced Retinal Degeneration and Regeneration in the Zebrafish: Histological and Functional Characteristics

Methylnitrosourea (MNU)-induced Retinal Degeneration and Regeneration in the Zebrafish: Histological and Functional Characteristics

DOI:
10.3791/51909-v

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08:53 min

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October 20, 2014

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, , , , ,
1Department of Ophthalmology, Inselspital,University of Bern, 2Department of Ophthalmology,University Hospital of Basel, 3Department of Biology,University of Fribourg

Chapitres

  • 00:05Titre
  • 01:35N‐methyl‐N‐nitrosourea Treatment
  • 02:35Visual Acuity Measurements
  • 03:57Histology
  • 05:12Immunohistochemstry
  • 07:01Results: Visual Acuity Testing and Retinal Degeneration/Regeneration Analysis
  • 08:16Conclusion

Summary

Traduction automatique

Traduction automatique

Herein we demonstrate quantification of retinal de- and regeneration and its impact on visual function using N-methyl-N-nitrosourea in the adult zebrafish. Loss of visual acuity and decreased photoreceptor numbers were followed by proliferation in the inner nuclear layer. Complete morphological and functional regeneration occurred 30 days after the initial treatment.

Tags

Retinal DegenerationRetinal RegenerationZebrafishN-methyl-N-nitrosourea (MNU)PhotoreceptorOptokinetic ReflexApoptosisProliferationHistologyImmunohistochemistry

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