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Methylnitrosourea (MNU)-induced Retinal Degeneration and Regeneration in the Zebrafish
 

Methylnitrosourea (MNU)-induced Retinal Degeneration and Regeneration in the Zebrafish: Histological and Functional Characteristics

Article DOI: 10.3791/51909-v • 08:53 min • October 20th, 2014
October 20th, 2014 •
1, 1,2, 1, 3, 3, 1
1Department of Ophthalmology, Inselspital, University of Bern, 2Department of Ophthalmology, University Hospital of Basel, 3Department of Biology, University of Fribourg

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Summary
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Herein we demonstrate quantification of retinal de- and regeneration and its impact on visual function using N-methyl-N-nitrosourea in the adult zebrafish. Loss of visual acuity and decreased photoreceptor numbers were followed by proliferation in the inner nuclear layer. Complete morphological and functional regeneration occurred 30 days after the initial treatment.

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Keywords: Retinal Degeneration Retinal Regeneration Zebrafish N-methyl-N-nitrosourea (MNU) Photoreceptor Optokinetic Reflex Apoptosis Proliferation Histology Immunohistochemistry
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