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Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants
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Journal JoVE Biologie
Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants

Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants

DOI:
10.3791/51945-v

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10:12 min

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September 02, 2014

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, , , , , ,
1Department of Horticulture and Landscape Architecture,Purdue University, 2Bindley Bioscience Center,Purdue University, 3Institute of Biotechnology,Jiangsu Academy of Agricultural Sciences, 4College of Environmental & Resource Science,Zhejiang University, 5Dryland Agriculture Research Centre,Shanxi Academy of Agricultural Sciences, 6Shanghai Center for Plant Stress Biology,Chinese Academy of Sciences

Chapitres

  • 00:05Titre
  • 01:45Aequorin Based Ca2+ Imaging Using the FAS (Film Adhesive Seedlings) System
  • 04:12Live Cell Confocal Ca2+ Imaging
  • 08:05Results: Measuring Spatial and Temporal Ca2+ Signals in Arabidopsis Plants
  • 09:39Conclusion

Summary

Traduction automatique

Traduction automatique

Ca2+ signaling regulates diverse biological processes in plants. Here we present approaches for monitoring abiotic stress induced spatial and temporal Ca2+ signals in Arabidopsis cells and tissues using the genetically encoded Ca2+ indicators Aequorin or Case12.

Tags

Calcium SignalingCa2+ ImagingAequorinCase12Plant BiologyLuminescenceConfocal Microscopy

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