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Chapitres
- 00:05Titre
- 02:20Common Carotid Artery and Distal Middle Cerebral Artery Occlusion by Ligation
- 03:56Perfusion and Sectioning of Brain Tissue
- 05:36Stereological Quantification of Infiltrated Neutrophils After Cerebral Ischemia by Optical Fractionation
- 07:23Brain Dissociation and Single Cell Suspension Preparation
- 09:41Results: Representative Flow Cytometric Analysis of Brain Leukocytes Obtained from Naïve, Sham and Ischemic Mice
- 11:44Conclusion
Brain myeloid cells characterization following stroke can be performed by stereology using the optical fractionator method, or by a flow cytometric analysis of brain leukocytes suspensions. Both are useful techniques to perform an accurate phenotypical distinction of the main myeloid cell subsets found in the ischemic brain.
